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The undifferentiated nucleotide specificities of specified archaeal ligases have tantalizing implications for enzyme evolution as well as the possible utilization of ADP like a substrate by ancestors of recent DNA ligases. The ability of some archaeal ligases to employ ATP and NAD+ Bet Ligaz or ATP and ADP as substrates may be discussed should they recognized only the ADP element prevalent to all a few nucleotides.
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Genetic deficiencies in human DNA ligases have been affiliated with medical syndromes marked by immunodeficiency, radiation sensitivity, and developmental abnormalities (three). The physiology and division of labor between cellular DNA ligases are the subjects of recent assessments (2–four) and won't be protected here. This minireview will target new insights to ligase system and evolution from ligase construction.
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